Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Sep 27 2007
Last update date: Mar 17 2012
Access: Public
Chemicals: Cocaine
Dataset link Genome-wide gene expression in HDAC5 wildtype and knockout mice treated with chronic cocaine or saline

Experimental Protocol


HDAC5 knockout mice and their wildtype littermate controls were injected i.p. with saline or 20 mg/kg cocaine for 7 days. Twenty-four hours after the last injection, mice were sacrificed and their NAc were dissected. NAc from four mice were pooled. Total RNA was extracted from each pool, labeled, and hybridized to Illumina Mouse 6 V1.1 full genome microarrays. For the first 12 microarrays, 8 mice/condition (WT saline, WT cocaine, KO saline, KO cocaine), were pooled into 2 groups, resulting in 4 mice per array and a total of two biological replicates and four technical replicates. For the second 18 microarrays, a newly treated set of 16 mice/condition were pooled into 4 groups for a total of 4 mice per array. This resulted in 4 more biological replicates per condition and 2 more technical replicates. In total, 30 microarrays yielded 6 biological replicates and 6 technical replicates (1-2 technical replicates per condition). The goal of this experiment was to identify gene expression differences between HDAC5 knockout and wildtype mice that may underlie their behavioral differences in response to cocaine.

Repositories


GEO

GSE9134

BioProject

PRJNA102673

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