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Benchling specifications

Information


Unique identifier OMICS_04729
Name Benchling
Interface Web user interface
Restrictions to use License purchase required
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Benchling Team <>

Additional information


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Benchling in publications

 (40)
PMCID: 5848568
PMID: 29530100
DOI: 10.1186/s13071-018-2762-3

[…] of the two genes were sequenced using sanger sequencing.table 1, the sequence data of the isolates were analysed using the clc genomics workbench 10.01 software (qiagen, aarhus, denmark) and benchling.com (california, ca, usa). pf3d7_1218300 and pf3d7_0104300 (plasmodb) were used as reference sequences to detect snps in the pfap2mu and pfubp1 respectively. poor quality sequences […]

PMCID: 5789018
PMID: 29379029
DOI: 10.1038/s41598-018-19784-2

[…] for crispr modification of the c-terminal end of the antibody, the region near the stop codon at the 3′ end of the exon encoding the c-terminus of the ch3 heavy chain was chosen for sgrna selection. benchling crispr sgrna design tool was used to select two sgrnas, one upstream and one downstream of the stop codon. sgrna 1 (agaaagctctcaggtcctaa) located 27 bp downstream of the end of stop codon […]

PMCID: 5785472
PMID: 29371699
DOI: 10.1038/s41598-018-20018-8

[…] advanced institute of science and technology (kaist). all cloning steps were carried out using e. coli top10 (invitrogen, us). identification of protospacer sequences in scen was performed using the benchling server. selected scen dna spacer was introduced into pcrispr-cas9 by ligating a pcr-generated sgrna sequence into snabi and ncoi linearized pcrispr-cas9 vector. to generate an 883 bp […]

PMCID: 5755516
PMID: 29286379
DOI: 10.3791/54703

[…] automation friendly protocols that are explicitly scheduled to run on liquid handling robots. though a number of software tools exist that allow researchers to design assemblies in-silico including benchling, moclo planner, and raven, few have the ability to translate those designs into executable instructions to run on a liquid handler. to that end, work such as pr-pr automation and puppeteer […]

PMCID: 5701044
PMID: 29170458
DOI: 10.1038/s41598-017-16279-4

[…] table ) were annealed and cloned into the digested plasmid., off-target and on-target scores were calculated using the ‘optimised crispr design tool’, available online by the zhang lab, mit 2013 and ‘benchling’s crispr tool’ available online by benchling., a dual luciferase assay was used to determine the potency and allele specificity of the different guides previously described. hek ad293 cells […]


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Benchling institution(s)
Benchling, Inc, CA, USA

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