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BeStSel specifications

Information


Unique identifier OMICS_12757
Name BeStSel
Alternative name Beta Structure Selection
Interface Web user interface
Restrictions to use None
Input data Wavelength and CD data
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline József Kardos <>

Publications for Beta Structure Selection

BeStSel in publications

 (37)
PMCID: 5895817
PMID: 29643444
DOI: 10.1038/s41598-018-24206-4

[…] the wavelength range from 190 nm-260 nm. the scanning speed was 100 nm/min, the bandwidth was 1 nm and the data pitch was 0.5 nm. the cd spectra were further analysed using the online software, bestsel, and the following proportion of different secondary structures were predicted., the cancer cell line h157 (atcc-crl-5802, atcc, teddington, middlesex, uk) and pc-3 (atcc-crl-1435, atcc, […]

PMCID: 5895000
PMID: 29641592
DOI: 10.1371/journal.pone.0194891

[…] is the molar residue ellipticity, θ is the cd signal in millidegrees, c is the protein concentration in μm, l is the pathlength in mm, and n is the number of residues. the cd data analysis program bestsel was used to analyze the data and estimate the secondary structure []., complementary dna oligonucleotides containing the trp120 gc-rich dna binding motif 5’-gcgattctcctgcctcagcctccctag-3’ […]

PMCID: 5937911
PMID: 29564502
DOI: 10.1007/s00216-018-0991-0

[…] mgcl2 was increased in the range of 0–100 mm to receive information about any changes in the secondary structure. the obtained spectra were further fitted with a method called β-structure selection (bestsel) that takes into account the twist of β-structures for estimation of the secondary structure. this method can reliably distinguish parallel and anti-parallel β-sheets and accurately estimate […]

PMCID: 5947572
PMID: 29430817
DOI: 10.1111/febs.14402

[…] far‐uv cd spectra (fig. a). the spectra did not change significantly upon addition of two molar equivalents of zncl2 or niso4, or replacement of nacl with kcl. inserting individual cd spectra into bestsel , a fold recognition algorithm, showed that the two variants contain similar helix and sheet content to both each other and the ctd of the 3d‐characterised e. coli homologue yiip (fig. b). […]

PMCID: 5817418
PMID: 29491709
DOI: 10.2147/IJN.S152742

[…] mean residue ellipticity [θ] was calculated as: [θ]=millidegreespathlength(mm)×proteinm×numberofresidues(3), a quantitative estimation of the protein secondary structures was obtained by using the bestsel method. for fourier transform infrared (ftir) measurements in attenuated total reflection (atr), 2 µl of 3.3 µm tz was deposited on the diamond plate of the single reflection atr device […]


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BeStSel institution(s)
Department of Biochemistry and MTA-ELTE NAP B Neuroimmunology Research Group, Institute of Biology, Eötvös Loránd University, Budapest, Hungary; DISCO Beamline, Synchrotron SOLEIL, Gif-sur-Yvette, France; Division of Protein Structural Biology, Institute for Protein Research, Osaka University, Osaka, Japan
BeStSel funding source(s)
This work was supported by the Hungarian Scientific Research Fund (K81950) and KTIA_NAP_13-2-2014-0017. SRCD measurements were supported by SOLEIL (Proposals 20140646, 20130475, 20120589, 201110054, and 20110405).

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