Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jul 27 2013
Last update date: Aug 23 2018
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Translational activation of developmental mRNAs during neonatal mouse testis development

Experimental Protocol


Extracts of mouse testes from 1 dpp and 4 dpp CD-1 mice were fractionated over a sucrose gradient in triplicate. Gradients were fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. The position of the 80S peak defined the boundary between translating and non-translating mRNAs. Pooled non-polysomal (non-translating; fractions 1-3) and heavy polysomal (efficiently translating, fractions 7-13) RNAs were isolated from sucrose gradient fractions for hybridization on Affymetrix 420 2.0 microarrays.

Repositories


GEO

GSE49283

ArrayExpress

E-GEOD-49283

BioProject

PRJNA213478

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Contact


Christopher Geyer
Christopher B Geyer