|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Jul 27 2013|
|Last update date:||Aug 23 2018|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Translational activation of developmental mRNAs during neonatal mouse testis development|
Extracts of mouse testes from 1 dpp and 4 dpp CD-1 mice were fractionated over a sucrose gradient in triplicate. Gradients were fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. The position of the 80S peak defined the boundary between translating and non-translating mRNAs. Pooled non-polysomal (non-translating; fractions 1-3) and heavy polysomal (efficiently translating, fractions 7-13) RNAs were isolated from sucrose gradient fractions for hybridization on Affymetrix 420 2.0 microarrays.
Christopher B Geyer