Computational protocol: MYCN gene expression is required for the onset of the differentiation programme in neuroblastoma cells

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Protocol publication

[…] After RA treatment, the cells were viewed with a Leica DM IRB inverted phase contrast microscope at × 200 (Leica, Wetzlar, Germany). The cells were scored as differentiated if the length of the neurite extensions was at least twice the diameter of the cell body. The neurites were evaluated based on the percentage of cells bearing neurites as determined from the cell culture images, which facilitated tracing the individual neurites and eventual branch points. A total of >300 cells was examined in five randomly chosen fields in each treated and untreated sample. The projection images were semiautomatically traced with NIH ImageJ using the NeuronJ plugin. The total dendritic length of each individual neuron was analysed. […]

Pipeline specifications

Software tools ImageJ, NeuronJ
Applications Phase-contrast microscopy, Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Neoplasms, Neuroblastoma