Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 21
Release date: Oct 4 2016
Last update date: Nov 27 2018
Access: Public
Dataset link BRD4 localization to lineage-specific enhancers is associated with a distinct transcription factor repertoire [mRNA-Seq]

Experimental Protocol


We performed mRNA sequencing from hFOB cells (undifferentiated and differentiated for 5 days into osteoblastic lineage) following BRD4 inhibition by JQ1 or siRNA mediated depletion. The mRNA-Seq includes namely 7 conditions: undifferentiated hFOBs treated with DMSO or non-targeting control siRNA (siCNTR), differentiated hFOBs with DMSO or siCNTR treatments; differentiated hFOBs treated with JQ1 or two siRNAs against BRD4 (#3 & #4). The libraries were performed in triplicates.

Repositories


GEO

GSE82289

ENA

SRP076175

BioProject

PRJNA324569

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