Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Sep 12 2013
Last update date: Aug 9 2018
Access: Public
Taxon: Homo sapiens
Dataset link Prostaglandin-modulated umbilical cord blood hematopoietic stem cells

Experimental Protocol


Isolated human CD34+ from umbilical cord blood were incubated ex vivo in Stem Span (SS) media evaluating three treatment temperatures (4 deg C, 25 deg C, and 37 deg C) with 10uM 16,16-dmPGE2 or Vehicle (DMSO) for 2 hours. To evaluate optimal media, similar CD34+ cells were incubated ex vivo in either Stem Span-SFEM (SS) media or 8% Low Molecular Weigh Dextran 40/5% HSA solution (LMD/HSA) with 10uM 16,16-dmPGE2 or Vehicle (DMSO) for 2 hours at 37 deg C. Total RNA was isolated post incubation and analyzed on Affymetrix microarrays for pathway activation.

Repositories


GEO

GSE46569

ArrayExpress

E-GEOD-46569

BioProject

PRJNA201017

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Contact


Dave Robbins