Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 20
Release date: Dec 18 2008
Last update date: Mar 16 2012
Access: Public
Diseases: Neoplasms, Neuroblastoma, Neuroendocrine Tumors
Chemicals: Amines, Amino Acids
Dataset link Identification of novel neuroendocrine markers by gene expression profiling

Experimental Protocol


The hybridisation was performed by an experienced laboratory technician Eli Helge, (Dept. of Laboratory Medicine, Children`s and Women`s health, NTNU) at the microarray core facility. Three separate runs with 10, 11 and 3 slides was needed to obtain the 20 hybridisations (dyeswap x 10 cell lines) planned for this study. Of the 10 cell lines 6 were neuroendocrine and 4 were non-neuroendocrine. For comparison between these two groups the universal human reference RNA (Stratagene) was used as the "control" on each slide. An adapted version of the 3DNA Array 350 protocol (Genisphere) was used. The slides were scanned with a ScanArrayTMExpressHT (Packard BioScience) twice immediately after the hybridisation was completed to avoid photo bleaching of the fluorescent dyes.

Repositories


GEO

GSE4328

ArrayExpress

E-GEOD-4328

BioProject

PRJNA94753

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Contact


Tom Wheeler
Tom Even Wheeler

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