Biosensor Processing statistics

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Citations per year

Number of citations per year for the bioinformatics software tool Biosensor Processing

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Biosensor Processing specifications


Unique identifier OMICS_26736
Name Biosensor Processing
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages MATLAB
Computer skills Advanced
Version 2.1
Stability Stable
Maintained Yes




No version available


  • person_outline Klaus Hahn

Publication for Biosensor Processing

Biosensor Processing citations


Calmodulin dissociates the STIM1 Orai1 complex and STIM1 oligomers

Nat Commun
PMCID: 5648805
PMID: 29051492
DOI: 10.1038/s41467-017-01135-w

[…] pped with high-speed fluorescence-external filter wheels for a CFP-YFP index-based FRET experiment. Images were collected every 10 s using a ×63 oil objective (N.A.1.4; Leica) and processed using the Biosensor Processing Software 2.1 package in MATLAB R2014a. FRET was determined by pixel-by-pixel calculation using apparent FRET efficiency calculation methods. Briefly, bleed-through and direct exci […]


RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development

PLoS One
PMCID: 5256999
PMID: 28114311
DOI: 10.1371/journal.pone.0170464

[…] P/FRET) filters for the collection of CFP and YFP fluorescence emission, respectively. Ratiometric FRET images of max intensity Z projections of the acquired CFP and YFP images were created using the Biosensor Processing Software 2.1 available from the Danuser laboratory (University of Texas Southwestern, Dallas, TX). Image analysis of the resulting FRET images was performed with Image J software. […]


Fibronectin Modulates Cell Adhesion and Signaling to Promote Single Cell Migration of Highly Invasive Oral Squamous Cell Carcinoma

PLoS One
PMCID: 4792484
PMID: 26978651
DOI: 10.1371/journal.pone.0151338

[…] th 2x digital zoom. Donor probe was excited with the 458nm laser line of an Argon ion laser (Melles Griot, Albuquerque, NM). Images were analyzed by Matlab® software (MathWorks, Natick, MA) using the Biosensor Processing software 2.1 []. The mean intensity values from FRET-ratio TIFF images were obtained on ImageJ software. Using ImageJ software, selected images were adjusted for the same levels o […]


Computer vision profiling of neurite outgrowth dynamics reveals spatiotemporal modularity of Rho GTPase signaling

PMCID: 4700477
PMID: 26728857
DOI: 10.1083/jcb.201506018

[…] o VC 60× (NA 1.4) objective (Nikon) in Neurobasal medium (Invitrogen). Förster resonance energy transfer (FRET) imaging was performed as described before (). Ratio imaging analysis was performed with Biosensor Processing Software 2.1 ( Images were thresholded on each channel; shade, background, and photobleach corrected; masked; and registered before rati […]


Spatially resolved intracellular sensing of hydrogen peroxide in living cells

Sci Rep
PMCID: 4653655
PMID: 26585385
DOI: 10.1038/srep16929

[…] o, Greifensee, Switzerland) before imaging () or 20 μL microloader tips (930001007, Eppendorf, Hamburg, Germany) during imaging (). Using MetaMorph (Molecular Devices, Sunnyvale, CA) and MatLab-based Biosensor Processing Software 2.1 (), the ratio of emission from 488 nm to that from 405 nm was calculated at each time point and represented in pseudocolor images.For , Structured Illumination Micros […]


ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity

PMCID: 4508895
PMID: 26169356
DOI: 10.1083/jcb.201504046

[…] c mirror/filter combination: SDM510/BA480-495 (CFP) and BA535-565 (YFP/FRET). Ratiometric FRET images of maximum intensity z projections of the acquired CFP and YFP/FRET images were created using the Biosensor Processing Software 2.1 available from the Danuser laboratory (University of Texas Southwestern, Dallas, TX). Image analysis of the resulting FRET images was performed with ImageJ software. […]

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Biosensor Processing institution(s)
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

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