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BioXTAS RAW specifications


Unique identifier OMICS_26458
Alternative name RAW
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Output data CSV
Operating system Unix/Linux, Mac OS, Windows
Programming languages C++, Python
License GNU General Public License version 3.0
Computer skills Medium
Stability Stable
Maintained Yes


No version available



  • person_outline Jesse Bennett Hopkins

Additional information

Publication for BioXTAS RAW

BioXTAS RAW citations


The C terminal region of translesion synthesis DNA polymerase η is partially unstructured and has high conformational flexibility

Nucleic Acids Res
PMCID: 5829636
PMID: 29385534
DOI: 10.1093/nar/gky031
call_split See protocol

[…] Pilatus3 1M detector at a sample-to-detector distance of 3.5 m. All data were collected at room temperature. Buffer subtraction was performed on all frames followed by evolving factor analysis using BioXTAS RAW to remove any trace contaminant peaks (). The 50 frames from the elution peak were averaged and used for all subsequent analysis. The elution profiles showing the frames used in averaging […]


Modular assembly of a protein nanotriangle using orthogonally interacting coiled coils

Sci Rep
PMCID: 5585338
PMID: 28874805
DOI: 10.1038/s41598-017-10918-6
call_split See protocol

[…] cattering data was collected at the G-line of the Cornell High Energy Synchrotron Source (G1), . Area Detector System CCD was used to collect the scattering patterns, and data analysis was done using BioXTAS RAW (version 1.0.0) to determine R g and molecular weight. […]


Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50

Sci Adv
PMCID: 5580885
PMID: 28879236
DOI: 10.1126/sciadv.1700532

[…] res of 2 s each were averaged for both protein-containing samples and background (buffer B only) samples. Buffer profiles were subtracted from the averaged protein solution profiles and reduced using BioXTAS RAW software (). Guinier analysis was performed using PRIMUS (, ), and the pair-distance distribution function P(r) and values for I0, Rg, and Dmax were obtained using the program GNOM (, ). R […]


Modulation of Interleukin 12 activity in the presence of heparin

Sci Rep
PMCID: 5509706
PMID: 28706183
DOI: 10.1038/s41598-017-05382-1
call_split See protocol

[…] A); beam diameter = 250 µm × 250 µm; photon flux = 1.6 × 1011 photons/sec. The detector used was a dual Pilatus 100K-S with a q-space range between 0.006 to 0.8 Å-1. Data acquired was processed using BioXTAS RAW software for performing the buffer subtraction. Buffer subtracted plots were analyzed using the ATSAS program with a sequence of steps to obtain an average low resolution structure. […]


Phosphorylation induces sequence specific conformational switches in the RNA polymerase II C terminal domain

Nat Commun
PMCID: 5437310
PMID: 28497798
DOI: 10.1038/ncomms15233

[…] rom a silver behenate standard was used for q-axis mapping. Data collection was performed using dual Pilatus 100K-S detectors. Reduction of the 2D images to 1D scattering profiles was performed using BioXtas Raw. Scattering profiles and uncertainties were computed as the average and s.d. of three exposures, with each exposure comprising 20 1-s frames. Solvent blanks were collected immediately befo […]


Structural heterogeneity in the intrinsically disordered RNA polymerase II C terminal domain

Nat Commun
PMCID: 5437306
PMID: 28497792
DOI: 10.1038/ncomms15231
call_split See protocol

[…] on, buffer subtraction, data reduction, Guinier analysis to a qRg<1.1, and molecular weight determination by extrapolation to I(0) and comparison to a glucose isomerase control were carried out using BioXtas RAW and plotted in Matlab. Buffer subtracted averaged scattering curves collected at three concentrations were averaged using PRIMUS, and distance distribution functions, Rg and Dmax were calc […]


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BioXTAS RAW institution(s)
Cornell High Energy Synchrotron Source, Ithaca, NY, USA; SAXSLAB ApS, Denmark

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