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Blast2GO specifications


Unique identifier OMICS_01475
Name Blast2GO
Alternative name Blast 2 GO
Software type Pipeline/Workflow
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
Computer skills Medium
Version 5.0
Stability Stable
Registration required Yes
Maintained Yes




No version available


Publications for Blast2GO

Blast2GO citations


Characterization of new microsatellite markers based on the transcriptome sequencing of Clematis finetiana

PMCID: 5952850
PMID: 29785177
DOI: 10.1186/s41065-018-0060-x

[…] no redundant (nr) protein database (ncbi), swiss prot database, kegg (kyoto encyclopedia of genes and genomes), cog (clusters of orthologous groups) and interpro database with an e-value<10− 5. blast2go software was used for gene ontology (go) annotation., the equivalent mixed rna samples (young leaves, stems and roots) from three individuals of c. finetiana which used for transcriptome […]


Comparative Genomics Reveals the Core Gene Toolbox for the Fungus Insect Symbiosis

PMCID: 5954228
PMID: 29764946
DOI: 10.1128/mBio.00636-18

[…] taxa was reconstructed using iq-tree, based on 29 proteins (longer than 50 aa) encoded by single-copy genes. the go names and subcellular protein locations of the fiscogs were predicted using blast2go v5.0 and the multiloc2 webserver against the fungal high-resolution database, respectively (, )., all four harpellales taxa and corresponding genome information have been deposited […]


Characterisation of proteins in excretory/secretory products collected from salmon lice, Lepeophtheirus salmonis

PMCID: 5948691
PMID: 29751843
DOI: 10.1186/s13071-018-2885-6
call_split See protocol

[…] involvement, signal peptide predictions were made from the translated cdna sequence representing each protein using signalp prediction software (cbs). identified proteins were investigated using the blast2go software suite []. sequences were blasted against the ncbinr database. proteins were then assigned into functional groups by searching the interpro databases and gene ontology databases. […]


Whole genome and transcriptome analysis reveal adaptive strategies and pathogenesis of Calonectria pseudoreteaudii to Eucalyptus

BMC Genomics
PMCID: 5946483
PMID: 29747580
DOI: 10.1186/s12864-018-4739-1

[…] uniref100-databases containing clustered sets of proteins from uniprot, pfam-database of protein families and kegg-database of metabolic pathways. go analysis of protein sequences were conducted by blast2go []. go enrichment was performed by cluego with p value < 0.05 []., to construct the phylogenomic tree of c. pseudoreteaudii and 13 other ascomycota isolates, including botrytis cinerea, […]


Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis

BMC Plant Biol
PMCID: 5941469
PMID: 29739322
DOI: 10.1186/s12870-018-1296-3

[…] minmatch, and minscore set to 0.95, 35, and 35, respectively, and all other parameters set to default. we used blast [] to align unigenes to nt, nr, cog, kegg and swissprot to obtain annotations, blast2go (version: v2.5.0; parameters: default) [] with nr annotation to obtain go annotations, and interproscan5 [] to obtain interpro annotations. clean reads were mapped to unigenes using bowtie2 […]


Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)

Sci Rep
PMCID: 5940786
PMID: 29739966
DOI: 10.1038/s41598-018-23874-6

[…] and, respectively. additionally, a kyoto encyclopedia of genes and genomes (kegg) pathway enrichment analysis was conducted using blast2go., protein spots with average intensities that did not significantly deviate from the mid-parent values (p > 0.05) were considered to correspond to additively accumulated proteins. […]

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Blast2GO institution(s)
Bioinformatics Department, Centro de Investigación Príncipe Felipe, Valencia, Spain
Blast2GO funding source(s)
Supported by the Spanish Ramon y Cajal Program and the National Institute of Bioinformatics (a platform of Genoma Espana).

Blast2GO reviews

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Josh Faber-Hammond

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Overall this program is easy to use, but I have many complaints about how commercial it has become. The free version has become virtually unusable for even relatively small NGS dataset due to speed and lack of very basic functionality (such as selecting sequences by name).
Above all else though, consider this a warning to anyone who wants to use the basic version for their analysis. BLAST2GO BASIC WITHHOLDS YOUR RESULTS AND DOESN'T NOTIFY YOU. I ran enrichment analysis side by side with the PRO and Basic versions of the software with the exact same input dataset and parameters. Enrichment analysis with the basic version gave me no significantly enriched GO categories, while the PRO version gave me many expected enriched GO categories for my dataset. Had I not done the side-by-side analysis I easily could have tried to publish false results showing that there were no significantly enriched GO categories in my dataset, and I never would have known otherwise.
Other functions are grayed out in the basic version, showing that you need to pay to gain access to that tool, while they leave a nonfunctional version of the enrichment test in there. While I generally can get behind entrepreneurship, Blast2GO's push for people to buy their PRO package is dishonest and may have led to the publication of countless false results. Who knows what other Blast2GO Basic functions are secretly withholding results. If anyone else is so inclined, please do your own tests to find out and let others know.

Amr Galal

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Blast2Go is very very great tool makes very much work in less time than using many different tools