Tool stats & trends
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|Alternative name||Blast 2 GO|
|Interface||Graphical user interface|
|Restrictions to use||License purchase required|
|Operating system||Unix/Linux, Mac OS, Windows|
No version available
- Documentation: https://www.blast2go.com/images/b2g_pdfs/b2g_user_manual.pdf
Publications for Blast2GO
Characterization of new microsatellite markers based on the transcriptome sequencing of Clematis finetiana
[…] using no redundant (Nr) protein database (NCBI), Swiss Prot database, KEGG (Kyoto Encyclopedia of Genes and Genomes), COG (Clusters of Orthologous Groups) and InterPro database with an E-value<10− 5. Blast2GO software was used for gene ontology (GO) annotation. […]
Comparative Genomics Reveals the Core Gene Toolbox for the Fungus Insect Symbiosis
[…] ncluded taxa was reconstructed using IQ-TREE, based on 29 proteins (longer than 50 aa) encoded by single-copy genes. The GO names and subcellular protein locations of the FISCoGs were predicted using Blast2GO v5.0 and the MultiLoc2 webserver against the fungal high-resolution database, respectively (, ). […]
Characterisation of proteins in excretory/secretory products collected from salmon lice, Lepeophtheirus salmonis
[…] involvement, signal peptide predictions were made from the translated cDNA sequence representing each protein using SignalP prediction software (CBS). Identified proteins were investigated using the Blast2Go software suite . Sequences were blasted against the NCBInr database. Proteins were then assigned into functional groups by searching the InterPro databases and Gene ontology databases. Anno […]
Whole genome and transcriptome analysis reveal adaptive strategies and pathogenesis of Calonectria pseudoreteaudii to Eucalyptus
[…] d uniref100-databases containing clustered sets of proteins from UniProt, Pfam-database of protein families and KEGG-database of metabolic pathways. GO analysis of protein sequences were conducted by Blast2GO . GO enrichment was performed by ClueGO with P value < 0.05 . […]
Identification of genes regulating ovary differentiation after pollination in hazel by comparative transcriptome analysis
[…] cy, minmatch, and minscore set to 0.95, 35, and 35, respectively, and all other parameters set to default. We used Blast  to align unigenes to NT, NR, COG, KEGG and SwissProt to obtain annotations, Blast2GO (Version: v2.5.0; parameters: default)  with NR annotation to obtain GO annotations, and InterProScan5  to obtain InterPro annotations. Clean reads were mapped to unigenes using Bowtie2 ( […]
Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)
[…] http://www.geneontology.org/ and http://www.expasy.ch/tools/pi_tools.html, respectively. Additionally, a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was conducted using Blast2GO. […]
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Above all else though, consider this a warning to anyone who wants to use the basic version for their analysis. BLAST2GO BASIC WITHHOLDS YOUR RESULTS AND DOESN'T NOTIFY YOU. I ran enrichment analysis side by side with the PRO and Basic versions of the software with the exact same input dataset and parameters. Enrichment analysis with the basic version gave me no significantly enriched GO categories, while the PRO version gave me many expected enriched GO categories for my dataset. Had I not done the side-by-side analysis I easily could have tried to publish false results showing that there were no significantly enriched GO categories in my dataset, and I never would have known otherwise.
Other functions are grayed out in the basic version, showing that you need to pay to gain access to that tool, while they leave a nonfunctional version of the enrichment test in there. While I generally can get behind entrepreneurship, Blast2GO's push for people to buy their PRO package is dishonest and may have led to the publication of countless false results. Who knows what other Blast2GO Basic functions are secretly withholding results. If anyone else is so inclined, please do your own tests to find out and let others know.