Blast2GO specifications


Unique identifier OMICS_01475
Name Blast2GO
Software type Pipeline/Workflow
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
Computer skills Medium
Version 5.0
Stability Stable
Registration required Yes
Maintained Yes



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Blast2GO articles

Blast2GO citations

PMCID: 5915582

[…] based on the best blast hit (highest score) along with their protein functional annotation., to annotate the gene with gene ontology terms, the nr database blast results were imported into the blast2go program53. this analysis mapped all of the annotated genes to go terms in the database and counted the number of genes associated with each term. perl script was then used to plot go […]

PMCID: 5954486

[…] (orf) size of 50 codons. the predicted proteome was annotated by similarity searches in the ncbi (national center for biotechnology information) nonredundant protein sequence (nr) database using the blast2go program (conesa et al., 2005). for comparative analysis, the h. irregulare tc 32‐1 genome and proteome sequences were downloaded from the jgi database (olson et al., 2012) and h. annosum […]

PMCID: 5859003

[…] to harbor their cds, where the cds of 11,975 unigenes were identified by blast alignment and that of 4603 were estimated by the estscan., to classify the predicted function of all unigenes, blast2go software was employed to search against go database and kog database. a total of 8425 unigenes were categorized into 57 functional groups under three go main categories, including […]

PMCID: 5853122

[…] million reads) method [24]. we used ‘fdr (false discovery rate) ≤ 0.001 and the absolute value of log2 rpkm ratio ≥ 1’ as the threshold to judge the significance of gene expression difference [25]., blast2go program was used to classify unigenes to go terms based on molecular function, biological processes and cellular components [26] for leaf and root tissues, at p < 0.05., qrt-pcr […]

PMCID: 5806860

[…] unigenes, 14607 unigenes were classified into three main categories namely, cellular components (3204, 21.93%), biological processes (5982, 40.95%) and molecular functions (5249, 35.93%) using blast2go software [19]. the go classifications are shown in s1 fig. among nine cellular components, cell (go: 0005623), cell part (go: 0044464), and organelle (go: 0043226) were the dominant terms. […]

Blast2GO institution(s)
Bioinformatics Department, Centro de Investigación Príncipe Felipe, Valencia, Spain
Blast2GO funding source(s)
Supported by the Spanish Ramon y Cajal Program and the National Institute of Bioinformatics (a platform of Genoma Espana).

Blast2GO reviews

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Josh Faber-Hammond

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Overall this program is easy to use, but I have many complaints about how commercial it has become. The free version has become virtually unusable for even relatively small NGS dataset due to speed and lack of very basic functionality (such as selecting sequences by name).
Above all else though, consider this a warning to anyone who wants to use the basic version for their analysis. BLAST2GO BASIC WITHHOLDS YOUR RESULTS AND DOESN'T NOTIFY YOU. I ran enrichment analysis side by side with the PRO and Basic versions of the software with the exact same input dataset and parameters. Enrichment analysis with the basic version gave me no significantly enriched GO categories, while the PRO version gave me many expected enriched GO categories for my dataset. Had I not done the side-by-side analysis I easily could have tried to publish false results showing that there were no significantly enriched GO categories in my dataset, and I never would have known otherwise.
Other functions are grayed out in the basic version, showing that you need to pay to gain access to that tool, while they leave a nonfunctional version of the enrichment test in there. While I generally can get behind entrepreneurship, Blast2GO's push for people to buy their PRO package is dishonest and may have led to the publication of countless false results. Who knows what other Blast2GO Basic functions are secretly withholding results. If anyone else is so inclined, please do your own tests to find out and let others know.

Amr Galal

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Blast2Go is very very great tool makes very much work in less time than using many different tools