BLASTP

[…] 2009). the dissimilarity in terms of protein diversity was analyzed and visualized in a distance tree. therefore, to identify homologous proteins shared between the genomes, an all-against-all blastp was performed, after which orthagogue v1.0.3 (ekseth et al. 2014) and mcl 14-137 (van dongen 2000) were used to identify orthologous groups of proteins (ogps), where the ogps included proteins […]

[…] 95% and coverage higher than 80% were cataloged as belonging to rescap database (rescapdb). the remaining sequences were translated to proteins. proteins were compared against uniprotkb database by blastp. again, hits with higher identity than 95%, coverage higher than 80% and e value lower than 10−100 were considered as uniprotkb known proteins. the set of remaining proteins […]

[…] pfam and uniprot databases, using trinotate and blast with a cut-off e-value of 10− 5. 21,259 (38.57%) unigenes were annotated at least in one database, including 6623 in nt, 18,556 in nr, 12,457 in blastp and 17,772 in blastx against uniprot (fig. 2a). on the basis of nr annotation, 15.53% (n = 2882) of unigenes matched to zootermopsis nevadensis, followed by acyrthosiphon pisum: 8.40% (n = 1560 […]

[…] and gene ontology databases. specifically, gene function annotations were based on the protein in the uniprot/swissprot database that had highest homology with the olympia oyster sequence (i.e., top blastp hit). gene targets were selected based on annotations related to gene regulation, immune response, and growth (table 1). corresponding contigs were then selected from the transcriptome using […]

[…] transporters and also set the stage for studies of an unusual mutant that may help elucidate mechanisms of cryptococcal pathogenesis and host response., uxt1 and uxt2 were identified by blastp searches of known nsts against c. neoformans predicted proteins (broad institute; cryptococcus neoformans var. grubii h99 database); the closest related sequence was that of the aspergillus […]