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Protocols

BlastStation specifications

Information


Unique identifier OMICS_32557
Name BlastStation
Alternative name BlastStation2
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
Computer skills Basic
Stability Stable
Maintained Yes

Subtools


  • BlastStation-Local64
  • BlastStation-Workgroup

Versioning


No version available

Maintainer


  • person_outline BlastStation Team

Additional information


https://www.patreon.com/blaststation

BlastStation citations

 (15)
library_books

Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont

2018
Nat Commun
PMCID: 5849685
PMID: 29535379
DOI: 10.1038/s41467-018-03366-x

[…] sequence identities >80% or >70% were removed from the agarose PUL or the porphyran PUL probe, respectively. The resulting trimmed probe sequences were used in subsequent BLAST searches using either BlastStation local 64 software or on IMG website. A metagenome was positive for a PUL if it returned two or more hits with ≥100 bp in length, ≥90% identity, and E-value ≤1 e−20. For metagenomic data s […]

call_split

Transcriptomic Analyses of Secreted Proteins From the Salivary Glands of Wheat Midge Larvae

2018
PMCID: 5822882
DOI: 10.1093/jisesa/iey009
call_split See protocol

[…] sequenced fully from both directions. If no overlap was found between the sense and antisense reads, new primers were synthesized for further sequencing.Cluster analyses of cDNAs were conducted using BlastStation-Local 64 program. Open reading frames (ORF) were identified using the ORF finder. Sequence alignment and similarity analysis were performed using various BLAST programs (http://www.ncbi.n […]

call_split

Developmental evolution facilitates rapid adaptation

2017
Sci Rep
PMCID: 5698356
PMID: 29162942
DOI: 10.1038/s41598-017-16229-0
call_split See protocol

[…] y the extracted loci, their sequences were aligned to the local STRING protein sequence database of Aspergillus nidulans (Emericella nidulans) (162425.protein.sequences.v10, http://string-db.org/) in BlastStation Local using BLAST (Basic Local Alignment Search Tool). These annotated proteins were submitted to AspGD GO-Slim: Process database (http://www.aspergillusgenome.org/cgi-bin/GO/goTermMapper […]

library_books

Selection and validation of appropriate reference genes for quantitative real time PCR analysis in Salvia hispanica

2017
PLoS One
PMCID: 5665522
PMID: 29091948
DOI: 10.1371/journal.pone.0186978

[…] psis.org/) database served as a query for a TBLASTN search against assembled transcripts of S. hispanica []. Local blast (TBLASTN) was done using the software given in the following link (https://www.blaststation.com/intl/en/blaststation2.php). The best hit for each gene was chosen based on the bit score and E-value. The 13 candidate reference genes include ACT11, EF1-α, ETIF3E, α-TUB, β-TUB, GAPD […]

call_split

Unravelling a stearidonic acid rich triacylglycerol biosynthetic pathway in the developing seeds of Buglossoides arvensis: A transcriptomic landscape

2017
Sci Rep
PMCID: 5585386
PMID: 28874672
DOI: 10.1038/s41598-017-09882-y
call_split See protocol

[…] as done with KAAS (KEGG Automatic Annotation Server) using the bi-directional best hit method against 15 plant reference organisms (see supplementary file ). Local BLAST (tBLASTN) was performed using BlastStation2 software (http://www.blaststation.com/intl/en/blaststation2.php). Protein sequences of Arabidopsis lipid genes were retrieved from TAIR (www.arabidopsis.org) and were used to query B. ar […]

library_books

Variation and evolution of polyadenylation profiles in sauropsid mitochondrial mRNAs as deduced from the high throughput RNA sequencing

2017
BMC Genomics
PMCID: 5576253
PMID: 28851277
DOI: 10.1186/s12864-017-4080-0

[…] ontaining the A or T stretches as a part of mtDNA sequences) increased considerably. We thus fixed the number of consecutive As/Ts to be 7 in the script.The blastn search (E-value cutoff: 1e-5) using BlastStation-Local 64 (TM Software) was conducted with the oligoA-containing reads as a database and the determined/deduced mtDNA sequence of the individual used for the RNA-Seq as a query. Reads that […]

Citations

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BlastStation institution(s)
TM Software, Inc., Arcadia, CA, USA

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