BLASTX pipeline

BLASTX specifications


Unique identifier OMICS_00992
Interface Web user interface
Restrictions to use None
Input data Accession numbers, gis, or sequences.
Input format FASTA, TXT
Computer skills Basic
Stability Stable
Maintained Yes


Publications for BLASTX

BLASTX citations

PMCID: 5945678

[…] a total of 46,556 assembled transcripts were obtained that were used for further analysis (supplementary table s3). the homology search of correctly assembled sequences was performed using blastx against non-redundant protein database of ncbi with e-value 10−5. out of 46,556 transcripts, 41,278 were found to have significant blastx hits, whereas no hits were found for 5,278 […]

PMCID: 5941679

[…] described above., all amplified products were sequenced using an abi3730xl automatic sequencer at sangon biotech (shanghai, china). similar sequences were searched using blastn for 16s rrna gene and blastx for arsb/acr3p. sequences from the isolates and reference sequences from genbank were checked manually and edited to the same lengths, using the software package clustalx 2.0 [23]. […]

PMCID: 5935750

[…] a specific dna fragment from only the targeted species or subspecies. the nucleotide sequences of each primer set were assessed for their specificity via ncbi blast modules, including blastn and blastx (, all conventional pcr reactions were performed in a total volume of 25 μl (1 × buffer, 0.2 mm of each dntp, 4.0 mm mgcl2) with 1.25 u of gotaq® […]

PMCID: 5891581

[…] encode putative obps and csps from the antennae, body transcriptomes and fourth instar larval transcriptome of b. odoriphaga (unpublished data). all putative obp and csp genes were confirmed by the blastx program at the national center for biotechnology information (ncbi, the open reading frames (orfs) of obp and csp genes were predicted by the orf […]

PMCID: 5896239

[…] onto the reference genome sequence. after assembly, we obtained a total of 45,109 transcripts with n50 of 2624 bp; the longest contig was 13,141 bp, and the average contig length was 1241 bp. blastx searches (e value <1e − 5) were performed against the refseq vertebrate other sequences in ncbi using blast2go [55]. we identified 35,456 transcripts that shared high homology with refseq […]

BLASTX institution(s)
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA

BLASTX review

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Dmitry Magas'

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Useful, but should have better UI