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Bleach Correction specifications


Unique identifier OMICS_25605
Name Bleach Correction
Alternative names CorrectBleach, Bleaching Correction
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java, Shell (Bash)
License GNU General Public License version 3.0
Computer skills Advanced
Version 2.0.2
Stability Stable
Maintained Yes




No version available


  • person_outline Curtis Rueden

Bleach Correction citations


Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Nat Commun
PMCID: 5682293
PMID: 29129912
DOI: 10.1038/s41467-017-01250-8

[…] For some data sets (mitochondria in Fig. ; Supplementary Movie ; microtubules in Fig. ; Supplementary Movie ), we performed standard bleaching correction based on an ImageJ Plugin ( some experiments, we observed a slight line artifact, obvious in maximum intensity projections of the raw data […]


TRIM9 dependent ubiquitination of DCC constrains kinase signaling, exocytosis, and axon branching

Mol Biol Cell
PMCID: 5576901
PMID: 28701345
DOI: 10.1091/mbc.E16-08-0594

[…] ll perimeter (0.64 μm in width), and the inner mask, defined as the remaining pixels that did not belong to the edge mask. DCC fluorescence intensity was corrected for photobleaching using the ImageJ CorrectBleach plug-in. Image stacks were temporally aligned to the “treatment frame,” at which time the cells were stimulated with netrin. The average pixel intensity for each region of interest (ROI; […]


Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum

PMCID: 5101878
PMID: 27877882
DOI: 10.1080/14686996.2016.1190258
call_split See protocol

[…] cquired following histamine treatment. The ratio of F340 to F387 was then calculated to show the response profile to histamine. In Figure (b), images were shown after photobleaching correction with a CorrectBleach plugin []. The p-value shown in Figure (c) was calculated by Student’s t-test. […]


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