Unlock your biological data

?

mass-spectrometry

Best bioinformatics software for mass-cytometry analysis

Conventional flow cytometry is limited by the number of fluorochromes and cell markers that can be targeted at once. Overcoming this limitation, mass-cytometry combines mass-spectrometry and flow cytometry by using metal-conjugated antibodies to label cellular proteins and extend the number of markers to be targeted.   A combination of spectrometry and cytometry   In mass-cytometry, antibodies are conjugated with isotopically pure elements, and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argonplasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in flow cytometry by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra.   Due to its numerous advantages (minimal …

Read this post
comment
0

Best bioinformatics software for MS-based proteomics analysis

Proteomics are the next step after genomics and transcriptomics to study biological systems. However, analyzing the proteome is much more difficult than the genome or transcriptome, because each cell expresses its own set of proteins. Mass spectrometry (MS) has emerged as the most important and popular tool to identify, characterize, and quantify proteins and their post-translational modifications with high throughput and on a large scale (Zhang et al.). To help you perform your experiments in the best conditions, we asked the OMICtools community to choose the best MS-based untargeted proteomics analysis tools. LC-MS based untargeted proteomics Two-dimension liquid-chromatography (LC) coupled with mass-spectrometry (LC-MS) is the leading technology for high-throughput proteomics. LC is used to separate proteins from different samples in parallel, and …

Read this post
comment
0

How to run proteomics analysis with mass-spectrometry

Proteins are an essential component of living organisms. In the systems biology era, the large-scale analysis of proteins and proteomes has a lot of applications, such as identification and quantification of proteins, study of post-translational modifications, protein structure, protein-protein or protein-nucleic acid interactions.   Here, we will focus on mass-spectrometry, the leading technology for high-throughput proteomics, and how it is used for protein characterization.  High-throughput proteomics Solving the complexity of gene expression at the whole-organism level with genomics and transcriptomics was thought to be the key to understanding the processes of life. However, protein expression does not always correlate with gene expression because mRNA is not always translated into protein, because a single transcript can code for several proteins, and …

Read this post
comment
0
< Previous 1 Next >