Breaking-Cas statistics

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Citations per year

Number of citations per year for the bioinformatics software tool Breaking-Cas

Tool usage distribution map

This map represents all the scientific publications referring to Breaking-Cas per scientific context
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Associated diseases


Popular tool citations

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Breaking-Cas specifications


Unique identifier OMICS_13525
Name Breaking-Cas
Interface Web user interface
Restrictions to use None
Input data One or several DNA sequences up to 20,000 nucleotides in total
Input format FASTA
Output data Rich interactive web page containing detailed information about candidate oligos, on-targets and off-targets. Scores, coordinates and overlapping genes are shown. Mini genome browsers allow checking the genomic environment of each putative off-target. Results can be downloaded as tables.
Programming languages Javascript, PHP
Computer skills Basic
Stability Stable
Maintained Yes



  • person_outline Florencio Pazos

Publication for Breaking-Cas

Breaking-Cas citation


Biased and Unbiased Methods for the Detection of Off Target Cleavage by CRISPR/Cas9: An Overview

Int J Mol Sci
PMCID: 5037784
PMID: 27618019
DOI: 10.3390/ijms17091507

[…] der (available online:, the CRISPR Design Tool ( [], E-CRISP (available online: [], and the Breaking-cas (available online: []. These algorithms have been used by several research groups to define the potential off-target sites. For example, C […]

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Breaking-Cas institution(s)
National Centre for Biotechnology (CNB-CSIC), c/ Darwin 3, Madrid, Spain; CIBERER, ISCIII, Madrid, Spain
Breaking-Cas funding source(s)
This work was supported by Spanish Ministry for Economy and Competitiveness (MINECO) [BIO2011-25687, BIO2012-39980, BIO2014- 57011-R].

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