Breaking-Cas statistics

To access cutting-edge analytics on consensus tools, life science contexts and associated fields, you will need to subscribe to our premium service.


Citations per year

Citations chart

Popular tool citations

chevron_left CRISPR/Cas9 sgRNA design chevron_right
Popular tools chart

Tool usage distribution map

Tool usage distribution map

Associated diseases

Associated diseases

Breaking-Cas specifications


Unique identifier OMICS_13525
Name Breaking-Cas
Interface Web user interface
Restrictions to use None
Input data One or several DNA sequences up to 20,000 nucleotides in total
Input format FASTA
Output data Rich interactive web page containing detailed information about candidate oligos, on-targets and off-targets. Scores, coordinates and overlapping genes are shown. Mini genome browsers allow checking the genomic environment of each putative off-target. Results can be downloaded as tables.
Programming languages Javascript, PHP
Computer skills Basic
Stability Stable
Maintained Yes



  • person_outline Florencio Pazos <>

Publication for Breaking-Cas

Breaking-Cas in publication

PMCID: 5404925
PMID: 28438259
DOI: 10.7554/eLife.21476.035

[…] chose showed very few off-targets in exonic regions and none in other or genes. we have now further applied two more tools to predict potential off-targets –cctop (stemmer et al. plos one 2015) and breaking-cas (olvieros et al. nar 2016) – and obtained 84 and 193 for the upstream grna (u-grna) and 132 and 206 for the downstream grna (d-grna), respectively:, , only three or genes […]

To access a full list of publications, you will need to upgrade to our premium service.

Breaking-Cas institution(s)
National Centre for Biotechnology (CNB-CSIC), c/ Darwin 3, Madrid, Spain; CIBERER, ISCIII, Madrid, Spain
Breaking-Cas funding source(s)
This work was supported by Spanish Ministry for Economy and Competitiveness (MINECO) [BIO2011-25687, BIO2012-39980, BIO2014- 57011-R].

Breaking-Cas reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review Breaking-Cas