Bright-field microscopy software tools
Bright field microscopy is a tried-and-tested method, relatively simple and widely used. The applications of image analysis with images acquired through bright field are many, from the correction of shading to the stitching of images to form a larger image to segmentation and measurement of cells or other objects of interest.
(Reyes-aldasoro., 2015) Bright field microscopy. Biomedical Image Analysis Recipes in MATLAB®: For Life scientists and Engineers.