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Btrim | An adapter and quality trimming program for next-generation sequencing technologies

A fast and lightweight software to trim adapters and low quality regions in reads from ultra high-throughput next-generation sequencing machines. It also can reliably identify barcodes and assign the reads to the original samples. Based on a modified Myers's bit-vector dynamic programming algorithm, Btrim can handle indels in adapters and barcodes. It removes low quality regions and trims off adapters at both or either end of the reads. A typical trimming of 30M reads with two sets of adapter pairs can be done in about a minute with a small memory footprint. Btrim is a versatile stand-alone tool that can be used as the first step in virtually all next-generation sequence analysis pipelines.

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Btrim classification

Btrim specifications

Unique identifier:
OMICS_01083
Interface:
Command line interface
Operating system:
Unix/Linux
Stability:
Stable
Software type:
Package/Module
Restrictions to use:
None
Computer skills:
Advanced
Maintained:
Yes

Btrim distribution

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Credits

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Publications

Institution(s)

Department of Molecular Biophysics and Biochemistry, WM Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT, USA

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