Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 31
Release date: May 17 2017
Last update date: Nov 26 2018
Access: Public
Diseases: Renal Insufficiency
Chemicals: Zinc
Dataset link KRAB-ZFP/KAP1 is essential to restrict excessive IGF2 expression during mouse embryo development

Experimental Protocol


ChIP-seq using WT and KO ESCs/TSCs for ZFP568 and H3K9me3. RNA-seq using WT and KO ESCs/TSCs and hybrid ESCs. ChIP-seq using IGF2 promoter crispr cell lines for ZFP568 and H3K9me3. Two platforms, Illumina Hiseq2500 and Solid 5500xL were used for sequencing. For Solid platforms, raw data was aligned to mm9 by LifeScopeā„¢ Genomic Analysis Software v2.5.1 to generate bam files. All data from Illumina platform was deposited in fastq format.

Repositories


GEO

GSE84832

ENA

SRP079880

BioProject

PRJNA335329

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Contact


Peng Yang