Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 44
Release date: Jun 10 2009
Last update date: Dec 17 2012
Access: Public
Dataset link Muscle gene expression of lambs with maternal callipyge allele

Experimental Protocol


10 lambs were used for this study. N=3 biological reps for C/C genotype, N=3 biological reps for Cmat/+ genotype, N=2 biological reps for +/Cpat genotype, and N=2 biological reps for +/+ genotype. Pairwise contrasts were performed to analyze the presence of a maternally inherited callipyge allele and the effect on a homozygous animal (+/C vs CC; +/+ vs C/+; and C/+ vs C/C). These data make up two experiments. [1] The maternal allele (4 genotype) experiment compares all four possible genotypes of a particular allele at one age and one tissue (30 days semimembranosus). [2] The paternal allele (2 genotype) experiment compares only the NC and the NN genotypes in one tissue (SM) across 4 ages (10, 20, 30, and 80 days). Images were interpreted with Microarray Suite version 5.0 (MAS5.0) in GCOS with no scaling or normalization (i.e., Samples GSM298143-GSM298164). Images were also interpreted by RMA using Bioconductor package of R (i.e., Samples GSM298165-GSM298186).

Repositories


GEO

GSE11780

ArrayExpress

E-GEOD-11780

BioProject

PRJNA105971

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Contact


Christopher Bidwell

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