|Dataset type:||Genome binding/occupancy profiling by high throughput sequencing, Epigenomics|
|Number of samples:||7|
|Release date:||Feb 4 2016|
|Last update date:||Oct 18 2018|
|Dataset link||Genome-wide analysis of ADP-ribosylation by ADPr-ChAP reveals its association with chromatin after oxidative stress|
Chromatin of untreated and H2O2-treated samples was pulled down with wild-type and R163A-mutant WWE domain. We performed ChAP followed by DNA-sequencinq of sheared chromatin from untreated cells enriched with wild-type RNF146 WWE domain to define the location of ADP-ribosylation in untreated cells, using the binding mutant as negative control. In addition, as oxidative stress is known to induce chromatin ADP-ribosilation, we include ChAP followed by DNA-sequencinq of sheared chromatin from H2O2-treated cells enriched with wild-type RNF146 WWE or the binding mutant.