Dataset features

Specifications


Dataset type: Genome binding/occupancy profiling by high throughput sequencing, Epigenomics
Number of samples: 7
Release date: Feb 4 2016
Last update date: Oct 18 2018
Access: Public
Dataset link Genome-wide analysis of ADP-ribosylation by ADPr-ChAP reveals its association with chromatin after oxidative stress

Experimental Protocol


Chromatin of untreated and H2O2-treated samples was pulled down with wild-type and R163A-mutant WWE domain. We performed ChAP followed by DNA-sequencinq of sheared chromatin from untreated cells enriched with wild-type RNF146 WWE domain to define the location of ADP-ribosylation in untreated cells, using the binding mutant as negative control. In addition, as oxidative stress is known to induce chromatin ADP-ribosilation, we include ChAP followed by DNA-sequencinq of sheared chromatin from H2O2-treated cells enriched with wild-type RNF146 WWE or the binding mutant.

Repositories


GEO

GSE69885

ENA

SRP059502

BioProject

PRJNA287023

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