Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Nov 1 2014
Last update date: Jul 26 2018
Access: Public
Diseases: Disorders of Sex Development
Dataset link Identification of candidate rhinovirus C (RV-C) receptors by gene expression analysis

Experimental Protocol


Sinus and bronchial epithelial tissue samples were obtained from residual surgical specimens. Primary airway epithelial cells were cultured submerged (undifferentiated monolayers) or at air-liquid interface (fully-differentiated). Established cell lines (HeLa, NCI-H358 and WisL) were cultured submerged. Epithelial tissue samples were placed in RNAlater solution (Life Technologies) to stabilize and protect cellular RNA; cultured cells were lysed directly and total RNAs were isolated using TRIzol reagent (Life Technologies). Gene expression was analyzed using the Human Genome 1.0 ST GeneChip arrays (Affymetrix, Santa Clara, CA).

Repositories


GEO

GSE61396

ArrayExpress

E-GEOD-61396

BioProject

PRJNA260942

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Contact


Yury Bochkov
Yury A Bochkov