|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Nov 1 2014|
|Last update date:||Jul 26 2018|
|Diseases:||Disorders of Sex Development|
|Dataset link||Identification of candidate rhinovirus C (RV-C) receptors by gene expression analysis|
Sinus and bronchial epithelial tissue samples were obtained from residual surgical specimens. Primary airway epithelial cells were cultured submerged (undifferentiated monolayers) or at air-liquid interface (fully-differentiated). Established cell lines (HeLa, NCI-H358 and WisL) were cultured submerged. Epithelial tissue samples were placed in RNAlater solution (Life Technologies) to stabilize and protect cellular RNA; cultured cells were lysed directly and total RNAs were isolated using TRIzol reagent (Life Technologies). Gene expression was analyzed using the Human Genome 1.0 ST GeneChip arrays (Affymetrix, Santa Clara, CA).
Yury A Bochkov