Dataset features
Specifications
Application: | Gene expression microarray analysis |
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Number of samples: | 8 |
Release date: | May 30 2013 |
Last update date: | Dec 29 2014 |
Access: | Public |
Diseases: | Retinal Neoplasms |
Dataset link | The polycomb repressive complex PRC2 regulates proliferation and neurogenesis in the Xenopus retina, and is governed by Wnt/beta-catenin signaling |
Experimental Protocol
Xenopus embryos were injected at the 8-cell stage with 5ng Ezh2 ATG MO or 5ng control MO (scrambled sequence of Ezh2 ATG MO) together with 400 pg mRNA for GFP as a lineage tracer. At stage 27, GFP-positive eyes were isolated by microdissection. Pools of 20-25 eyes were used to prepare total RNA for each sample on the microarray. 4 control and 4 Ezh2 ATG MO samples were hybridized to Agilent 1-color microarrays.
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Brett Milash