Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: May 30 2013
Last update date: Dec 29 2014
Access: Public
Diseases: Retinal Neoplasms
Dataset link The polycomb repressive complex PRC2 regulates proliferation and neurogenesis in the Xenopus retina, and is governed by Wnt/beta-catenin signaling

Experimental Protocol


Xenopus embryos were injected at the 8-cell stage with 5ng Ezh2 ATG MO or 5ng control MO (scrambled sequence of Ezh2 ATG MO) together with 400 pg mRNA for GFP as a lineage tracer. At stage 27, GFP-positive eyes were isolated by microdissection. Pools of 20-25 eyes were used to prepare total RNA for each sample on the microarray. 4 control and 4 Ezh2 ATG MO samples were hybridized to Agilent 1-color microarrays.

Repositories


GEO

GSE47456

ArrayExpress

E-GEOD-47456

BioProject

PRJNA205635

Download


Contact


Brett Milash