Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Dec 22 2011
Last update date: Jul 1 2016
Access: Public
Chemicals: Zinc
Dataset link Transcriptional targets of Trps1 in vibrissae

Experimental Protocol


Whole whisker pads were dissected from E12.5 embryos and total RNA was isolated using the RNeasy Mini Kit (Qiagen). Triplicate RNA samples from three independent embryos of each genotype were amplified and labeled for hybridization to Affymetrix GeneChip MOE430A microarrays using Affymetrix reagents and protocols. The data output was analyzed using GeneSpring GX 10.0 commercial software (Agilent Technologies). P-values were calculated using an unpaired t-test. Expression values with a p-value less than or equal to 0.05 and a fold difference of at least 1.5 relative to wild-type baseline expression levels were considered significant.

Repositories


GEO

GSE33766

ArrayExpress

E-GEOD-33766

BioProject

PRJNA148375

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Contact


Katherine Fantauzzo