Dataset features


Application: Gene expression microarray analysis
Number of samples: 95
Release date: Nov 17 2010
Last update date: Jan 17 2017
Access: Public
Diseases: Alzheimer Disease, Genital Diseases, Female, Hyperaldosteronism, Neoplasms, Ovarian Diseases, Ovarian Neoplasms, Neoplasms, Second Primary
Chemicals: Platinum
Dataset link Gene expression profiles of ovarian cancer cell lines in the presence and absence of a DNA methyltransferase inhibitor

Experimental Protocol

The objective of the study was to identify genes that are subject to DNA methylation through pharmacological inhibition of DNA methyltransferase activity in a panel of cancer cell lines. Cells were mock treated with culture media (mock treated) or treated with 5 µM decitabine for 72 hours. Resulting expression profiles were compared to identify genes with altered expression following decitabine treatment. These data represent two experiments: In the first, 43 established cell lines were mock treated or treated with decitabine to enable identification of genes differentially expressed as a result of inhibition of DNA methyltransferase activity. HEYA8-decitabine treated cells were run in replicate. In the second experiment, A2780 and PEO1 cells underwent flow activated cell sorting to separate CD133(+) from CD133(-) cells in each cell line; the sorted cell populations were cultured in the same manner as the first experiment and similarly mock treated or treated with decitabine. All specimens were arrayed in parallel and used for RMA normalization.