Insights into function and regulation of microRNAs by decoding degradation dynamics [4SU]

To obtain a global picture of miRNA decays, we performed high-throughput sequencing analysis (Illumina) of labeled miRNAs along the “pulse and chase” experiment. A total of 21 libraries were produced, using 4SU RNA isolated from 7 time points and 3 independent biological replicas. We additionally sequenced i) total RNA from growing cells (not labeled without extraction – UNT), ii) total RNA pulsed with 4SU (labeled without extraction – 4SU); and iii) 4SU RNA from non-labeled cells (not labeled with extraction, negative control – NEG).