Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 24
Release date: Jan 20 2016
Last update date: Oct 10 2018
Access: Public
Diseases: Extensively Drug-Resistant Tuberculosis
Dataset link Insights into function and regulation of microRNAs by decoding degradation dynamics [4SU]

Experimental Protocol


To obtain a global picture of miRNA decays, we performed high-throughput sequencing analysis (Illumina) of labeled miRNAs along the “pulse and chase” experiment. A total of 21 libraries were produced, using 4SU RNA isolated from 7 time points and 3 independent biological replicas. We additionally sequenced i) total RNA from growing cells (not labeled without extraction – UNT), ii) total RNA pulsed with 4SU (labeled without extraction – 4SU); and iii) 4SU RNA from non-labeled cells (not labeled with extraction, negative control – NEG).

Repositories


GEO

GSE72655

ENA

SRP063106

BioProject

PRJNA294601

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Contact


Heiko Muller