Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: May 2 2006
Last update date: Oct 29 2018
Access: Public
Diseases: Deficiency Diseases, Neoplasms
Dataset link MCF10A cells in 3-D culture for 15 h after treatment with CtIP-RNAi vs Luc-RNAi adenovirus

Experimental Protocol


MCF10A cells seeded at 5x105 cells/60mm plate were infected with adenoviral luciferase- or CtIP-RNAi in duplicate at 20 MOI for 24h. Infected cells were re-seeded at 5x105 cells in a 60mm plate pre-coated with Growth Factor Reduced Matrigel and covered with the growth medium containing 2% Matrigel at 37°C for 15h. RNA was extracted and quality assessed. cDNA synthesized from the harvested RNA was biotin-labeled, hybridized onto Affymetrix HG U133 PLUS 2.0 array (54,676 genes) and stained with streptavidin-phycoerythrin. The hybridized array was analyzed using GeneChip® Scanner 3000 and GCOS 1.2 software (Affymetrix) at the UCI Microarray Core service.

Repositories


GEO

GSE4751

ArrayExpress

E-GEOD-4751

BioProject

PRJNA104543

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Contact


Wen-Hwa Lee