Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Sep 1 2007
Last update date: Jan 25 2018
Access: Public
Diseases: Chemical and Drug Induced Liver Injury
Chemicals: Estradiol, Estrogens, Glucose
Dataset link Molecular targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the zebrafish ovary

Experimental Protocol


A total of eight samples were analyzed including three TCDD doses (10, 40, 100 ppb, dietary exposure) and vehicle control samples. cRNA targets were synthesized from pooled total RNA isolated from five ovaries from different individuals in each treatment group. Each individual contributed equally to a given pool. Two technical replicates were run for each treatment group. Several genes of interest arising from this microarray study were validated by Quantitative RT-PCR with individual ovary samples to assess biological variability.

Repositories


GEO

GSE4859

ArrayExpress

E-GEOD-4859

BioProject

PRJNA95833

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Contact


Craig Struble