|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Sep 1 2007|
|Last update date:||Jan 25 2018|
|Diseases:||Chemical and Drug Induced Liver Injury|
|Chemicals:||Estradiol, Estrogens, Glucose|
|Dataset link||Molecular targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) within the zebrafish ovary|
A total of eight samples were analyzed including three TCDD doses (10, 40, 100 ppb, dietary exposure) and vehicle control samples. cRNA targets were synthesized from pooled total RNA isolated from five ovaries from different individuals in each treatment group. Each individual contributed equally to a given pool. Two technical replicates were run for each treatment group. Several genes of interest arising from this microarray study were validated by Quantitative RT-PCR with individual ovary samples to assess biological variability.