Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Nov 17 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Brain Ischemia
Mutations: rs7956957
Dataset link Expression data from human blood samples

Experimental Protocol


Blood was extracted from 12 subjects. EDTA tubes were centrifuged at 3500rpm for 15 min to obtain the white blood cell fraction and the samples were stored at -80ºC until RNA isolation. RiboPureTM -Blood kit from Ambion (Ambion, Woodward st. Austin, USA) was used to extract total RNA following manufacturer’s instructions. Globin RNA from erythrocytes that causes interference was extracted using the Globin-Clear kit (Ambion, Woodward st. Austin, USA).

Repositories


GEO

GSE25414

ArrayExpress

E-GEOD-25414

BioProject

PRJNA134161

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Contact


Sophie Domingues