Computational protocol: Semi Rolled Leaf2 modulates rice leaf rolling by regulating abaxial side cell differentiation

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Protocol publication

[…] The amino acid sequences of the SRL2 homological proteins were retrieved through a database search using the total amino acid sequences of SRL2. The multiple sequence alignments were performed by Clustal_X 1.83 () using the parameters of Weight matrix: Gonnet; Gap opening penalty: 10.0 and Gap extension penalty: 0.10. Phylogenetic trees were constructed with the aligned protein sequences using MEGA 3.1 software () based on the Maximum likelihood method with parameters of the Poisson correction model, complete deletion, and bootstrap (1 000 replicates; random seed). […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Oryza sativa
Diseases Mucolipidoses