Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Jan 24 2014
Last update date: Nov 9 2017
Access: Public
Diseases: Neoplasms, DNA Repair-Deficiency Disorders
Dataset link Expression data of BRCA1 shRNA and PTEN shRNA single knockdown, BRCA1_PTEN shRNA double knockdowns and control shRNA in MCF-10A cells

Experimental Protocol


Various shRNAs that target genes involved in homologous recombination (HR) were transfected in MCF-10A non-transformed breast cells lines. Stable HR gene knockdown MCF-10A cells were seeded 200000 at 10 cm plate. Cells were harvested after 48 hours culturing and used for gene expression profiling. The shRNAs that target PTEN or BRCA1 genes were transfected in MCF-10A non-transformed breast cell line by lentiviral particles to generate either single gene knockdown or double gene knockdown. Stable BRCA1, PTEN, and BRCA1_PTEN MCF-10A cells were selected. Scrambled control shRNA-transfected MCF-10A cells were applying as control. All knockdown and control MCF-10A cells were seeded with 2 x 10^5 cells at 10 cm culture plate. Cells were cultured in MCF-10A medium and harvested after 48 hours culturing. mRNA was extracted from collected cells and performing gene expression profiling. Three or four biological replicates were applied. Four biological replicates were applied.

Repositories


GEO

GSE54265

ArrayExpress

E-GEOD-54265

BioProject

PRJNA236216

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Contact


Chun-Jen Lin
Chun-Jen Curtis Lin

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