Computational protocol: Mutation detection analysis of a region of 16S-like ribosomal RNA gene of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii

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Protocol publication

[…] PCR products of 16S-like rRNA genes of E. histolytica, E. dispar and E. moshkovskii showing mutation by riboprinting or SSCP analysis, were sequenced on a ABI3730XL sequencer (Macrogen, Seoul, South Korea) using species specific primers. The nucleotide sequences were edited with reference to chromatographs using Chromas (Version 1.62) software and aligned using BioEdit (version 7). […]

Pipeline specifications

Software tools Chromas, BioEdit
Application Sanger sequencing
Organisms Entamoeba histolytica, Entamoeba dispar, Homo sapiens
Diseases Liver Abscess