Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Apr 11 2009
Last update date: Aug 28 2018
Access: Public
Dataset link ngatha mutant inflorescences

Experimental Protocol


RNA was extracted from inflorescences of 30 day old plants using Qiagen RNAEasy kit. cRNA was synthesized and hybridized to Affymetrix ATH1 array according to manufacture recommendation. Seven repeats of wild type, two repeats of nga quadruple mutants and two repeats of 35S:amiR-NGA164a were collected. Signal values were obtained and normalized using the GC-RMA protocol, using R 2.7.2 (www.r-project.org) and Bioconductor 2.2 (www.bioconductor.org/). Average correlation values between repeats was 0.985, while the average correlation between wild type and mutant samples was only 0.972. As the average correlation between samples the nga quadruple mutants and 35S:amiR-NGA164a plants was 0.984, and given the identical phenotype, we grouped the two genotypes together.

Repositories


GEO

GSE15555

ArrayExpress

E-GEOD-15555

BioProject

PRJNA116001

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Contact


Idan Efroni
Idan D. Efroni