Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Nov 15 2008
Last update date: Aug 28 2018
Access: Public
Chemicals: Progesterone
Genes: PTTG, Apg1
Dataset link Gene expression analyses of PR action in the mammary gland of ovariectomized mice

Experimental Protocol


Microarray analysis was performed on mammary tissue total RNA obtained from ovariectomized mice previously treated with progesterone for 4, 16, 28, and 76 hours. For each hormone treatment time-point, mammary gland microarray data from sesame oil (vehicle) treated mice was included as controls. Total mammary gland RNA from each time-point and treatment group was interrogated using the Affymetrix GeneChip Mouse Genome 430 2.0 Array. For a given time-point and treatment group, three sets of mice (5 mice in each set) were used for gene expression profiling; mammary RNA pooled from each set was hybridized to one microarray chip. Therefore, a total of 15 mice (or 3 microarray chips) were used per time-point and treatment group.

Repositories


GEO

GSE10290

ArrayExpress

E-GEOD-10290

BioProject

PRJNA108501

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Contact


Chad Creighton