Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 52
Release date: Dec 26 2016
Last update date: Dec 26 2016
Access: Public
Chemicals: Glucose
Dataset link Sequential global gene expression analysis of glucose stimulated human islets

Experimental Protocol


We performed gene expression profiling on samples of human pancreatic islets from 5 donors stimulated by 300 mg/dl glucose for 1 hr (5 biological repeats), 2 hr (3 biological repeats) and 24 hr (5 biological repeats) for a total of 13 arrays. For comparison, gene expression profiling was conducted on unstimulated pancreatic islets for 1 hr (4 biological repeats), 2 hr (5 biological repeats) and 24 hr (4 biological repeats) for a total of 13 arrays. Single samples of fibroblasts were treated for 1 hr and 24 hr and untreated for 1 hr, 2 hr and 24 hr periods for a total of 5 arrays. Hepatocytes were treated for 1 hr (1 sample), 2 hr (1 sample) and 24 hours (2 technical repeats) and untreated for 1 hr (1 sample), 2 hr (2 technical repeats) and 24 hr (2 technical repeats) for a total of 9 arrays. PBMC were treated for 1 hr (2 technical repeats), 2 hr (2 technical repeats) and 24 hr (2 technical repeats) and untreated for 1 hr (2 technical repeats), 2 hr (2 technical repeats) and 24 hrs (2 technical repeats) for a total of 12 arrays. Total RNA from PBMCs pooled from six normal donors was amplified into aRNA to serve as the reference.

Repositories


GEO

GSE24555

BioProject

PRJNA132647

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Contact


Francesco Marincola