|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||Jun 1 2012|
|Last update date:||Jul 26 2018|
|Diseases:||Hodgkin Disease, Lymphoma, Non-Hodgkin, Neoplasms|
|Genes:||FOXO1, CDKN1B, PMAIP1, BCL2L11, NCX, FBXO32, CBLB, CCL5, CXCR5, TNFRSF8|
|Dataset link||Expression data of cHL cell lines after FOXO1 activation|
KM-H2, L428, L1236, UH0-1, and SUP-HD1 cells expressing constitutively active mutant of human FOXO1 fused in frame with estrogen receptor ligand-binding domain were incubated with 200 µcle (ethanol). After 24 h, total RNA was isolated with RNeasy mini kit (QIAGEN). Microarray analyses were performed using 200 ng of total RNA as starting material and 5.5 ug ssDNA per hybridization (GeneChip Fluidics Station 450; Affymetrix, Santa Clara, CA). The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (http://www.affymetrix.com). Labeled ssDNA was hybridized to Human Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA). The chips were scanned with a Affymetrix GeneChip Scanner 3000 and subsequent images analyzed using Affymetrix Expression Console Software (Affymetrix). Probe level data were obtained using the robust multichip average (RMA) normalization algorithm.