Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Jun 26 2009
Last update date: Mar 20 2012
Access: Public
Diseases: Infection
Chemicals: Calcium, Iron, Glutamic Acid
Dataset link Microarray Analysis of Acid-Stress Induced Changes in EHEC O157:H7

Experimental Protocol


Bacteria were grown in LB broth overnight, then subcultured into DMEM and grown at 37C, 5%Co2. Bacteria were then subjected to one of three acid stress protocols: 1) UA30: growth in DMEM pH 7.4 followed by growth in DMEM pH 3.0 for 30 minutes; 2) AA30: growth in DMEM pH 5.0 (adaptation) followed by growth in DMEM pH 3.0; 3) UA15: growth in DMEM pH 7.4 followed by growth in DMEM pH 3.0 for 15 minutes. DMEM was supplemented with 25 mM MES (pH 5.0) and in the case of the control (unadapted, unshocked) 25 mM MOPS (pH 7.4) and the adaptation step was again carried out at 37C and 5% CO2. Acid shocking was done at pH 3.0 (unbuffered) at room temperature for all treatments

Repositories


GEO

GSE14069

ArrayExpress

E-GEOD-14069

BioProject

PRJNA110365

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Contact


Debora Foster