Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 54
Release date: Dec 11 2012
Last update date: Jul 26 2018
Access: Public
Genes: MIR107
Dataset link Specific sequence determinants of miR-15/107 microRNA gene group targets

Experimental Protocol


RIP-ChIP studies (and parallel assessments of total input mRNA) were performed in cultured H4 cells after transfection with miRNAs corresponding to the miR-15/107 gene group (miR-103, miR-107, miR-16 and miR-195), another physiological miRNA (miR-320), miR-15b*, and three non-physiological additional miRNA-like molecules (miR-107-mut1, mir-107-mut2, and negative control miRNA from Ambion). For details on transfected miRNA sequences, see Nelson PT et al, Nucleic Acids Res. 2011 Oct;39(18):8163-72. Three biological replicates were run for each condition with a total of 54 separate human Affymetrix Human Gene 1.0 ST array replicates. See Wang WX et al, RNA (2010) 16:394-404 for detailed protocol of RIP-ChIP.

Repositories


GEO

GSE42823

ArrayExpress

E-GEOD-42823

BioProject

PRJNA183527

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