Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jul 13 2011
Last update date: Jul 26 2018
Access: Public
Dataset link Expression data from human naïve (TN), stem cell memory (TSCM), central memory (TCM) and effector memory (TEM) CD8+ T cells

Experimental Protocol


Three healthy human blood donors provided lymphocyte-enriched apheresis blood for this study after informed consent. From all samples, total RNA was isolated using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Human Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix), all according to the respective manufacturer's instructions. Samples represent "exon-level" and "gene-level" analyses.

Repositories


GEO

GSE23321

ArrayExpress

E-GEOD-23321

BioProject

PRJNA131239

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Contact


Zoltan Pos

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