Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 16
Release date: Oct 22 2011
Last update date: Mar 23 2012
Access: Public
Taxon: Homo sapiens
Dataset link Human bone marrow precursor cells

Experimental Protocol


Total RNA from cell populations highly enriched in precursors from three succesive stages of neutrophil granulopoiesis and mature neutrophils isolated from bone marrow and peripheral blood, respectively, from four healthy donors (SKP, AJ, AO, JO). The three cell populations from bone marrow where extracted and named B3, B2, and B1, corresponding to immature (myeloblasts [MBs] and promyelocytes [PMs]); intermediate mature (myelocytes [MCs] and metamyelocytes [MMs]); and mature neutrophil cells (band cells [BCs] and segmented neutrophil cells [SCs]), respectively, as described in Bjerregaard MD, Jurlander J, Klausen P, Borregaard N, Cowland JB: The in vivo profile of transcription factors during neutrophil differentiation in human bone marrow. Blood 2003, 101: 4322-4332. In total, 16 samples were compared (4 cell maturation stages from 4 donors)

Repositories


GEO

GSE33140

ArrayExpress

E-GEOD-33140

BioProject

PRJNA149387

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Contact


Corinna Pedersen
Corinna Cavan Pedersen