Computational protocol: Three Mitogen-Activated Protein Kinases Required for Cell Wall Integrity Contribute Greatly to Biocontrol Potential of a Fungal Entomopathogen

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Protocol publication

[…] The sequences of Bck1, Mkk1 and Slt2 (NCBI codes: EJP69353, EJP70226 and AEU60018 respectively) were blasted from the Bb2860 genome under the NCBI accession ADAH00000000 via BLASTP analysis (http://blast.ncbi.nlm.nih.gov/blast.cgi) using the queries of homologous proteins of several fungi in NCBI protein database. Their full-length gene sequences were amplified from Bb2860 via PCR with paired primers () and sequenced at Invitrogen for verification. The proteins deduced from the verified sequences were structurally compared with homologues in other fungi, followed by phylogenetic analysis with MEGA5 . […]

Pipeline specifications

Software tools BLASTP, MEGA
Databases NCBI Protein
Application Phylogenetics
Organisms Saccharomyces cerevisiae, Beauveria bassiana
Diseases Larva Migrans
Chemicals Glycerol, Mannitol, Sorbitol, Trehalose