Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: May 3 2012
Last update date: Jul 6 2016
Access: Public
Chemicals: Ciprofloxacin, Propidium, Tobramycin
Dataset link Pseudomonas aeruginosa biofilm cells contain ribosome hibernation factors in the dormant antibiotic-tolerant subpopulation

Experimental Protocol


52-hour Pseudomonas aeruginosa TSA colony biofilms were cryoembedded, thin sectioned, and laser dissected (LCM) to obtain samples from the top and bottom 50 µm of the biofilms. 9 sections per biofilm were pooled. RNA was extracted with the RNeasy Micro kit, Turbo DNase treated, poly(A) tailed, and amplified using the Quantitect WTA kit. After clean up, the resulting product was fragmented and end labeled before hybridization.

Repositories


GEO

GSE34762

ArrayExpress

E-GEOD-34762

BioProject

PRJNA150357

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Contact


Kate McInnerney