|Application:||Gene expression microarray analysis|
|Number of samples:||10|
|Release date:||May 3 2012|
|Last update date:||Jul 6 2016|
|Chemicals:||Ciprofloxacin, Propidium, Tobramycin|
|Dataset link||Pseudomonas aeruginosa biofilm cells contain ribosome hibernation factors in the dormant antibiotic-tolerant subpopulation|
52-hour Pseudomonas aeruginosa TSA colony biofilms were cryoembedded, thin sectioned, and laser dissected (LCM) to obtain samples from the top and bottom 50 µm of the biofilms. 9 sections per biofilm were pooled. RNA was extracted with the RNeasy Micro kit, Turbo DNase treated, poly(A) tailed, and amplified using the Quantitect WTA kit. After clean up, the resulting product was fragmented and end labeled before hybridization.