Computational protocol: Discovery of a Novel hsp65 Genotype within Mycobacterium massiliense Associated with the Rough Colony Morphology

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Protocol publication

[…] Determined partial rpoB (711-bp), and hsp65 (603-bp) sequences were aligned using the ClustalW algorithm in MEGA4 . A phylogenetic tree based on the rpoB and hsp65 gene sequences was constructed by the neighbor-joining and maximum-parsimony methods within the MEGA 4 program . The constructed neighbor-joining tree was evaluated by bootstrap value calculated from 1,000 replicates . Separately, the 23 S rRNA gene sequences of 65 M. massiliense clinical isolates were analyzed to observe any point mutation at the adenine at position 2058 (A2058) or at A2059 in the peptidyltransferase region of the 23 S rRNA gene . […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Homo sapiens
Diseases Mycobacterium Infections