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Specifications


Application: Gene expression microarray analysis
Number of samples: 5
Release date: Nov 21 2008
Last update date: Mar 20 2012
Access: Public
Taxon: Fundulus heteroclitus
Dataset link Technical Analysis of Fundulus heteroclitus cDNA Microarrays, experiment A

Experimental Protocol


mRNA expression was measured using microarrays where each array had four spatially separated replicates per gene. The 384 F. heteroclitus cDNA microarrays were printed using 55 control genes and 329 cDNAs which encode essential proteins for cellular metabolism. To test for the relationship between fluorescence and the quantity of RNA, five concentrations of fluorescently labeled RNA were used: 1.2 to 700 pmol of Cy3 or Cy5. A 15 µl hybridization using the 384 cDNA array corresponds to 0.09 to 47 µM of Cy dye. Cy5 dye labeled RNA was used at concentrations 18% less than Cy3 because the Cy5 dye is a more efficient fluorophore (greater fluorescence per photon) than the Cy3 dye. The average of eight fluorescence values for each gene was normalized to the original concentration of RNA added.

Repositories


GEO

GSE12858

ArrayExpress

E-GEOD-12858

BioProject

PRJNA114169

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Contact


Cinda Scott
Cinda P Scott

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