Computational protocol: Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12

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Protocol publication

[…] The phylogenetic relationship of CHI II was generated with 24 other deduced chitinases available from the NCBI databases as shows in Table . A phylogenetic tree was constructed by multiple sequence alignment using clustal X [] and was generated using the Neighbour-Joining method (NJ) and bootstrap analysis. The phylogenetic tree was visualised using Treeview software. Confidence values for individual branches were assessed from 1000 bootstrap replicates of the original sequence data. […]

Pipeline specifications

Software tools Clustal W, TreeViewX
Application Phylogenetics
Organisms Komagataella pastoris
Chemicals Methanol