Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: Sep 23 2017
Last update date: Nov 30 2018
Access: Public
Chemicals: Nucleotides, Thiouridine
Dataset link Thiol-linked alkylation for the metabolic sequencing of RNA [Transcriptional output measurement by SLAM-seq in wildtype mES cells]

Experimental Protocol


Mouse embryonic stem (mES) cells were subjected to 45 min s4U-pulse labeling followed by total RNA extraction, alkylation, mRNA 3' end library preparation (Quant-seq, Lexogen) and high throughput sequencing.

Repositories


GEO

GSE99972

ENA

SRP109171

BioProject

PRJNA390466

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Contact


Brian Reichholf