Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Nov 1 2007
Last update date: Jul 6 2016
Access: Public
Taxon: Caenorhabditis elegans
Dataset link cRNA amplification methods enhance microarray identification of transcripts expressed in the nervous system

Experimental Protocol


We profiled gene expression throughout the nervous system of the model organism Caenorhabditis elegans using two different amplification methods, WT-Pico and IVT (in vitro transcription). Since postembryonic cells are unattainable via this method, we used mRNA-tagging to isolate pan-neural RNAs from larval animals. In short, an epitope tagged poly-A binding protein (FLAG-PAB-1) was driven in all neurons using the F25B3.3 promoter. We then amplified harvested RNA using IVT or WT-Pico. Data generated from this study reveals significant differences between the two amplification methods. However, both seem to identify bona fide neural transcripts. Our work suggests that using two amplification methods, when possible, will provide one with a more accurate view of transcription in a particular cell type.

Repositories


GEO

GSE9485

ArrayExpress

E-GEOD-9485

BioProject

PRJNA103265

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Contact


David Miller

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