|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||2|
|Release date:||Sep 22 2010|
|Last update date:||May 23 2013|
|Dataset link||Discovery and comparative profiling of microRNAs in sweet oranges|
Size fractionated small RNAs (16-30 bp) from total RNA extracts was ligated to 5' and 3' adapters, and reverse transcribed. After PCR amplification the sample was subjected to Solexa sequencing. The resultant 35nt sequence data were filtered according to base quality value. The remained sequences were used to trim 5' and 3' adaptors. The clean tags were used for further analysis.