|Application:||Gene expression microarray analysis|
|Number of samples:||56|
|Release date:||Oct 1 2015|
|Last update date:||Aug 6 2018|
|Dataset link||The regional mouse microglial transcriptome during aging|
In this study 4, 12 and 22 month old mice were transcardially perfused with 0.9% saline. Brains were collected and dissected into 4 regions: cerebellum, cortex, hippocampus, striatum. For the extraction of microglia tissue from 8 mice was pooled for each regional replicate and the experiment was performed in quadruplicates for each region. Microglia were extracted from each region using a magnetic bead based approach. Brain regions (2 animals per region) of 4 month old animals for total RNA extraction of homogenates were snap frozen and stored at (-80C). Total RNA was immediately isolated for purified microglia and stored (-80C) until performing microarray analysis of purified microglia and regional brain homogenates (n=56).
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