Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 56
Release date: Oct 1 2015
Last update date: Aug 6 2018
Access: Public
Diseases: Hypersensitivity, Immediate
Dataset link The regional mouse microglial transcriptome during aging

Experimental Protocol


In this study 4, 12 and 22 month old mice were transcardially perfused with 0.9% saline. Brains were collected and dissected into 4 regions: cerebellum, cortex, hippocampus, striatum. For the extraction of microglia tissue from 8 mice was pooled for each regional replicate and the experiment was performed in quadruplicates for each region. Microglia were extracted from each region using a magnetic bead based approach. Brain regions (2 animals per region) of 4 month old animals for total RNA extraction of homogenates were snap frozen and stored at (-80C). Total RNA was immediately isolated for purified microglia and stored (-80C) until performing microarray analysis of purified microglia and regional brain homogenates (n=56).

Repositories


GEO

GSE62420

BioProject

PRJNA264067

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Contact


Barry McColl

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